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KMID : 0900919940180010025
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Korean journal of Animal Reproduction
1994 Volume.18 No. 1 p.25 ~ p.29
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Studies on the In-Vitro Fertilization and Survival Rate of Rapidly Frozen Porcine Immature Embryos
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Jung Young-Ho
Kim Sang-Keun
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Abstract
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This study was carried out to investigate the in-vitro fertilization rate survival rate of rapidly frozen porcine immature embryos. The porcine embryos following dehydration by cryoprotectants containing sucrose were directly plunged into liquid nitrogen and thawed in 30^{circ}C water bath. Survival rate was defined as development rate on vitro culture or FDA test. The results are summarized as follows : 1. The in vitro fertilization rate of all frozen immature oocytes(6.7~26.7%) was very low, 35.0% of unfrozen oocytes and the rate of immature oocytes was very higher than that of mature oocytes. 2. The survival rate of all frozen immature oocytes(10.3~25.0%, 13.3~30.0%) was very low, 45% of unfrozen oocytes and the rate of immature oocytes was slightly higher than that of mature oocytes
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